Propagation of asparagus racemosus through tissue culture. Micropropagation of asparagus densiflorus via axillary. Senpropagation of asparagus racemosus through tissue culture. In vitro propagation of a high value medicinal plant. Asparagus racemosus due to its slow growth faces lots of problem which can be solved through tissue culture.
Supplementing asparagus racemosus will aid digestion, since the plant has antiulcer effects. Rapid in vitro multiplication of asparagus sciencedirect. Asparagus racemosus have numerous medicinal usages which have been reported in the indian and british pharmacopoeias and in indigenous systems of medicine. Induction of somatic embryos in cultures of asparagus. Murashige and skoogs medium with 2, 4d and kinetin induced callus in the shoot segments of asparagus racemosus. Uses, benefits, cures, side effects, nutrients in asparagus racemosus. Shatavari asparagus racemosus is a wondrous plant that holds a special place in the heart of the ayurvedic pharmacopoeia. Studies using seeds as means of propagation indicated. Pdf asparagus racemosus commonly known as shatavari in marathi and hindi. This plant is known to produce steroidal saponins called shatavarins. Asparagus officinalis cultivar franklim was successfully propagated in vitro on a.
Little work has been undertaken on in vitro culture in asparagus racemosus as compared to that of a. Asparagus leaf extract inhibits growth of renal cell cancer cells asian pac j cancer prev, 15 5, 19371941 introduction asparagus racemosus willd. Micropropagation of asparagus racemosus shatavari asparagus racemosus shatavari is a plant with immense medicinal values. Asparagus racemosus, traditionally known as shatavari means who possesses a hundred husbands or acceptable to many. Since the callus quality plays an important role in plantlet development, therefore, compact embryogenic callus was selected for further embryogenesis. Pdf micropropagation and phytochemical analysis of asparagus. The therapeutic applications of this plant have been reported in indian and british pharmacopoeias and in traditional system of medicine, such as ayurveda, unani and siddha. Pdf micropropagation of asparagus by in vitro shoot culture. Asparagus racemosus is one of the important medicinal plants found in india, china and other parts of the world. In indian system of medicine asparagus racemosus is an important medicinal plant and its root paste or root juice has been used in various ailments and as health tonic 1,2.
Micropropagation of asparagus densiflorus via axillary shoots, indirect organogenesis and somatic embryogenesis anna pindel a. In vitro clonal propagation of asparagus racemosus, a high. It is good source of folic acid, potassium, dietary fiber. Micropropagation of asparagus densiflorus via axillary shoots, indirect organogenesis and somatic embryogenesis. A induction of calli on shoot tips and nodal bud explants on ms medium supplemented with 4 mg l1 bap and 0. Pdf asparagus officinalis is most extensively studied species within the genus asparagus, which is well known as garden asparagus. It will also aid the immune system when antibodies are fighting off a threat. A significant increase in antibody titers was observed compared to animals that were untreated. Asparagus racemosus is an herb used in ayurveda medicine. Asparagus racemosus willd is multiplied through vegetative propagation in nature. The amounts of saponin equivalent to shatavarin iv in the extracts were determined using elisa. Asparagus racemosus herb uses, benefits, cures, side. A read is counted each time someone views a publication summary such as the title, abstract, and list of authors, clicks on a figure, or views or downloads the fulltext. Shatavarin production from in vitro cultures of asparagus.
Micropropagation insures a good regular supply of medicinal plants, using minimum space and time prakash and staden, 2007. Asparagus racemosus satavar, shatavari, or shatamull, shatawari is a species of asparagus common throughout india and the himalayas. Study of phytohormones effect on micropropagation and shoot induction in asparagus racemosus int j cur res rev, nov 2012 vol 04 22 page 48 in case of reproductive disease. Investigation of cytotoxicity and invitro antioxidant activity of asparagus racemosus root extract. Conservation micropropagation introduction asparagus racemosus willd.
The multiple uses of this species have increased its commercial demand, resulting in overexploitation. In the present study, somatic embryogenesis via zygotic embryos was studied in asparagus racemosus willd. Teratogenicity of asparagus racemosus roots 571 methanol 1 l by cold percolation method for 5 days. In vitro propagation of wild asparagus adscendens roxb. There is always problem of heterogeneity in obtaining uniform seed stock. B adventitious shoot formation from surface of calli on ms with 2 mg l1 and 0. Asparagaceae, commonly called shatavari, is a woody climber that grows throughout tropical and subtropical parts of india up to an altitude of 1,500 m. Learn more about asparagus racemosus uses, effectiveness, possible side effects, interactions, dosage, user ratings and products that contain asparagus racemosus. Shatavari, also known as satavari, satavar, or asparagus racemosus a. Hence, there is an urgent necessity to safeguard this germplasm, and plant tissue culture has proved helpful in conserving threatened plant species. Thanks to its cooling, soothing, nourishing, and rejuvenating work throughout the body, shatavari effectively balances both pitta and vata.
Its medicinal usage has been reported in the indian and british pharmacopoeias and in traditional systems of medicine such as ayurveda, unani, and siddha. In spite of being a rejuvenating herb it is beneficial in female infertility, as it increases libido. R2 1department of biotechnology, school of life sciences, vels. It is reported to be recalcitrant to tissue culture practices.
Pdf micropropagation and phytochemical analysis of. List of various diseases cured by asparagus racemosus. Mohommad shahid department of botany, davpg college, dehradun. Conservation of medicinal plant asparagus racemosus.
Names of asparagus racemosus in various languages of the world are also given. How asparagus racemosus is effective for various diseases is listed in repertory format. Micropropagation of asparagus by in vitro shoot culture. Asparagus officinalis is most extensively studied species within the genus asparagus, which is well known as garden asparagus. Asparagus racemosus leaf extract inhibits growth of uok. Part of the methods in molecular biology book series mimb, volume 547 asparagus racemosus willd. Asparagus racemosus supplement health benefits, dosage. Somatic embryogenesis is one of the most popular in vitro regeneration methods for mass micropropagation.
Regeneration of shoot buds and clonal multiplication of excised shoots through proliferation of nodal buds could be achieved by the use of iaa and bap in the medium. The plant is also known as shatavari which means she who has hundred husbands which indicates to the rejuvenative effect. It has antioxidant activity, antiabortifacient activity shatvarin 1, antioxytoxic shatavarin4, spasmodic to uterus, hypoglycemic. Liliaceae is a pharmacologically acclaimed phytoestrogenic medicinal plant used for its immunomodulatory diwanay et al. Asparagus racemosus is a well known ayurvedic rasayana which prevent ageing, increase longevity, impart immunity, improve mental function, vigor and add. Rapid in vitro micro propagation of asparagus racemosus willd. Shatavari, micropropagation, tissue culture, in vitro culture. The roots are said to be tonic and diuretic and galactgogue, the drug has ulcer healing effect probably via strenthening the mucosal resistance or cytoprotection. Shatavari asparagus racemosus is a powerful therapeutic agent, which is of great importance in ayurveda. Asparagus racemosus a medicinally important plant can be propagated through tissue culture technique which save lots of time and money. Rapid in vitro protocol for high multiple shoot induction.
Kar dk and sen s 1985 propagation of asparagus racemosus through tissue culture. This species is dioecious with unisexual flowers, which means that generative propagation gives roughly equal number of male and female plants. Asparagus racemosus is a commercially important medicinal plant, traditionally used for combating gynecological problems in india. Micropropagation of asparagus racemosus shatavari abstract. It contains various type of secondary metabolite active natural products or ingredients such as steroids, alkaloids, flavonoids, furan derivatives and essential oils. The majority of plants used by the pharmaceutical industry come from wild sources, endangering the natural population of the species.
The plants are being overharvested, so this species faces a real danger of becoming vulnerable in its natural habitat. Regeneration of asparagus racemosus by shoot apex and. Direct in vitro propagation of asparagus adscendens. Asparagus racemosus roots were successively extracted with the series of solvents, that is, hexane, ethanol and water, and also a saponin. The multiple uses of this species have increased its commercial demand. The extracts were filtered, vacuum dried and stored in a refrigerator until further use. Asparagus racemosus an overview sciencedirect topics. Asparagus racemosus is an indigenous medicinal plant of the family liliaceae 1956.